Interpretation of CID Mass Spectra

Professors: Jack Henion, Ph.D., Emeritus Professor of Analytical Toxicology
Status: Registration open

Summary:
Course Instructor:

Jack Henion, Ph.D., Emeritus Professor of Analytical Toxicology, Cornell University

 Course Syllabus:

This one-day relatively advanced course covers concepts for interpreting the collision-induced dissociation mass spectra of small molecules such as drugs, impurities, degradants, environmental contaminants, etc.  The course includes six (6) one-hour lectures including extensive problem solving.  The prospective student should have a good understanding of organic chemistry, reaction mechanisms, and the guidelines of physical organic chemistry.  An understanding of EI mass spectral interpretation is also helpful.

 

Who Should Attend

Researchers, practitioners, or others involved in identifying compounds detected in real-world samples including drugs, metabolites, contaminants and toxicants.  Scientists who are interested in the agrochemical, industrial, toxicological or chemical fields will also benefit from this course, although the focus is on small molecule pharmaceutical compound problems.

 

The course is designed as a practical introduction with considerable practice in problem solving which should enable the interested student to apply the basic rules of mass spectral interpretation for characterizing unknown compounds.  It is desirable for the student to be familiar with the basics of physical organic chemistry reaction mechanisms, atmospheric pressure ionization principles, and tandem mass spectrometry.

 

Key Topics You Will Learn

·         Strategies for approaching CID mass spectral interpretation

·         The importance of acquiring high-quality CID mass spectral information for interpretation purposes

·         The nitrogen rule, importance of stable isotope contributions, mass defects, the Retro Diels Alder reaction, accurate mass measurement, high resolution and related topics.

·         Differentiation between simple bond cleavages, alpha cleavages, rearrangements, and ring opening mechanisms

·         Rules for approaching an unknown structure characterization given a good CID mass spectrum

·         Relevance of data from triple quadrupole MS systems, Ion Trap MS, LC-TOF/MS, FTMS, Orbitraps, MS/MS and other modern analytical information

 How You Will Benefit from This Course

·         Gain insight to approaching your analytical problems from a leading expert

·         Review the basics and parallels between the interpretation of EI mass spectra compared with CID mass spectra

·         Learn specific LC/MS and LC/MS/MS techniques for characterizing or identifying components in complex samples

·         Perform numerous problem solving exercises from applications in the environmental, biotechnology, pharmaceutical, chemical industries, and chemical terrorism including trace analyses

·         Pick up helpful suggestions and hints to help solve your own analytical problems

 

About the Instructor

Jack Henion is Professor Emeritus of Toxicology at Cornell University in the Analytical Toxicology Section of the Diagnostic Laboratory within the New York State College of Veterinary Medicine.  He is also a co-founder, Chairman, and CSO of Advion BioSciences, Inc., located in Ithaca, New York. Prof. Henion has published extensively in the areas of conventional capillary GC/MS as well as LC/MS, SFC/MS, IC/MS, and CE/MS using atmospheric pressure ionization (API) technologies with quadrupole, ion trap, FTMS, and time-of-flight mass spectrometers.