Courses

After Congress Course: Mass Spectrometry for Proteomics - 16-17 dec

Professors: Simon J. Gaskell (Manchester) & Robert J. Beynon (Liverpool)
Status: Registration open

Summary:

Mass Spectrometry for Proteomics

SHORT COURSE: 16 -17 DECEMBER 2009

Establishing the function of gene products is the major challenge of the post genomic era …the rate-limiting step will be the speed with which proteins can be isolated, identified, characterized and quantified.

This short course taught by faculty members of the University of Manchester (UK) and the University of Liverpool (UK) will provide a thorough introduction to the analysis of proteins and peptides by mass spectrometry and will appeal to mass spectrometrists and biochemists seeking to work at the leading edge of modern protein characterisation and proteomics

Course Description and Aims

Extraordinary developments made in the last decade have dramatically extended the application of mass spectrometry to the analysis of biopolymers. Mass spectrometry is now an essential component in the analytical armament of the protein and peptide scientist. This course has been taught, twice yearly, at The University of Manchester since 1995 (www.mbc.manchester.ac.uk/events/) to great acclaim in Europe. The course program is regularly reviewed and updated to reflect the rapid progress in this dynamic scientific field. The course program covers two and a half days (02-03 May 2007) with lectures/seminars and problem solving exercises. Course instruction will be by lecture/seminar with the emphasis on practical methods and problem solving. There will be ample opportunity to discuss individual problems with the course tutors. All participants will be provided with a comprehensive course manual as an enduring reference source.

Programme Outline

· Mass spectrometry in Proteomics Research· Principles of Tandem Mass Spectrometry (MS/MS)· Interpretation of Tandem Mass Spectra of Polypeptides· Protein Identification & Optimising Protein Database Searching· Characterization of Glycoproteins/Phosphoproteins· Top-Down & Bottom-Up Proteomics Approaches· Quantitative Proteomics Strategies (LC/MS/MS)· Work-Flow Design for Proteomics Research

Course Tutors

Professor Simon J. Gaskell V.P. Research, The University of Manchester Director, Michael Barber Centre for MS, The School of Chemistry, University of Manchester, UK.

Professor Robert J. Beynon Director, The Protein Function Group Faculty of Veterinary Science, University of Liverpool, UK

This short course is made available through sponsorship by Waters Corporation

Mass spectrometry for proteomics SHORT COURSE (BRAZIL) 16-17 DECEMBER 2009

Day 1: Wednesday 16^th December

09.00 Introduction

09.15 1. Complexity of the Protein World (RJB)10.00 2. Introduction to mass spectrometry of proteins and peptides - A (SJG)11.00 Break11.15 3. Tandem MS (SJG)

12.45 Discussion

13.00 Lunch

14.00 4. Developing A Proteomics Workflow (RJB)

15.00 Break

15.30 5. Analysis of intact proteins (RJB)16.00 6. Analysis of non-covalent interactions (SJG)

17.00 Discussion

Day 2: Thursday 17^th December

09.00 7. Quantitative proteomics using in vitro labelling (SJG)10.00 8. Quantitative proteomics by metabolic labelling in vivo (RJB)

11.00 Break

11.30 9. Quantitative Proteomics with label-free Techniques (vitro/in vivo) (MMcD)

12.30 Discussion13.00 Lunch14.00 10. Post-translational modifications (SJG)15.00 11. ION MOBILITY – A NEW DIMENSION IN TOP-DOWN/BOTTOM-UP PROTEOMICS (MMcD)