MALDI Imaging: Principles and Applications & Current Methods in Mass Spectrometry for Proteomics

Professors: Prof. Dr. Carlos Bloch Jr; Gongyi Shi, Ph.D; Christian Klein Ph.D
Status: Registration open

Summary:
Title: MALDI Imaging: Principles and Applications
Time: 8am to 12pm
Speaker: Prof. Dr. Carlos Bloch Jr. - Laboratory of Mass Spectrometry, Embrapa Genetic Resources and Biotechnology .- Embrapa

Title: "Current Methods in Mass Spectrometry for Proteomics Research": Basic, yet Comprehensive Introduction of the Basic Concepts in Proteomics
Time: 13h to 17h
Speaker: Gongyi Shi, Ph.D.; MALDI Applications Manager - Bruker Daltonics
Christian Klein Ph.D., Senior Applications Scientist - Bruker Daltonics


Imaging mass spectrometry is a recent technique whose foundations are basically the same as the conventional method. The set of procedures known as MS Imaging includes visualization of the spatial distribution of peptides, proteins, metabolites, and any ionizable molecule present in slices of biological tissues compatible with existing detection systems used in mass spectrometry. In the specific case of MALDI MS Imaging, a saturated solution of the appropriate matrix must be applied either manually or automatically on the tissue to enable the best possible ionization of the molecules of interest. The mass spectrometers prepared for such experiments are MALDI-TOF equipped with lasers (commonly in the UV region), preferably of solid phase that can reach frequencies up to 1 kHz and greater durability than that of traditional nitrogen lasers. These spectrometers have to register the distribution of ions obtained from a tissue area previously marked by the operator. After completion of the acquisition, data should be processed with the help of a dedicated imaging software. Both proprietary and open softares can be used to visualize and compare the results with images from light microscopy.

                 This course will present the fundamentals of the technique of MALDI Imaging, examples and solutions explored by the group of the Laboratory of Mass Spectrometry of Embrapa Genetic Resources and Biotechnology related to the steps of sample preparation, acquisition and interpretation of data obtained from tissue animals and plants.